FIGURE 5. The maturation of β₂ integrin activation during the DMSO-induced differentiation of HL60 cells.

(A) Contour plots showing expression of high-affinity (H⁺, mAb24 staining) and extended (E⁺, KIM127 staining) β₂ integrins on HL60 cells (upper panels) or primary human neutrophils (lower panel) stimulated with fMLP (1 mM, right panels) or vehicle control (left panels). HL60 cells were pre-differentiated with 1.3% DMSO for indicated days. (B,C) Expression of mAb24 (B) and KIM127 (C) on HL-60 cells after different days of DSMO-induced differentiation with or without fMLP stimulation. (D) The percentage of mAb24⁺KIM127⁺ HL-60 cells after different days of DSMO-induced differentiation with or without fMLP stimulation. (E) Expression of overall CD18 (conformation unspecific) on HL-60 cells after different days of DSMO-induced differentiation with or without fMLP stimulation. (F) FPR1 expression on HL-60 cells after different days of DSMO-induced differentiation. Mean ± 95%CI for n = 3 individual experiments in (B-G), **P < 0.01, ***P < 0.001, ****P < 0.0001 by comparing fMLP stimulated cells and vehicle control using 2-way ANOVA followed by Sidak’s multiple comparisons test