Figure 1.

The hrpA promoter activity of MS2 in LS5 medium supplemented with different concentrations of salicylic acid (SA; A), p-hydroxybenzoic acid (PHBA; B), cinnamyl alcohol (CA; C), p-coumaric acid (PCA; D), and hydrocinnamic acid (HA; E) at 14 h of growth. MS2(pPhrpA-gfp) cultures (OD₆₀₀ of 2.0) were transferred into the LS5 medium supplemented with different concentrations of tested compounds or DMSO at a ratio of 1:10, and then dispended into a 96 well-tissue culture plate (100 μl per well) for cultivation in the dark for 14 h. MFI was measured by a Multifunctional Microplate Reader. DMSO was used as the positive control. Three replicates were used in each compound and three independent experiments were performed with similar results. For statistical analysis, GraphPad Prism 8.4.3 software was used to perform Student’s t-test. Asterisks indicate statistically significant differences (^*p < 0.05, ^**p < 0.01, ^***p < 0.001, and ^****p < 0.0001).