Table 2.
The hrpA promoter activity of MS2 in LS5 medium supplemented with optimal concentrations of compounds at 14 h of growth and the corresponding inhibition rates of the compounds toward hrpA promoter activity.
| Multifunctional Microplate Reader | Flow cytometer | |||||
|---|---|---|---|---|---|---|
| Compound | MFI ± SDa | DMSO%b | Inhibition rate% (100%-DMSO%) | MFI ± SDa | DMSO%b | Inhibition rate% (100%-DMSO%) |
| DMSO | 16,605.33 ± 75.61 | / | / | 11,520.60 ± 349.49 | / | / |
| 0.15 mM SA | 6,660.67 ± 35.16 | 40.11 | 59.89 | 2,201.97 ± 177.32 | 19.11 | 80.89 |
| 0.4 mM PHBA | 7,792.67 ± 108.04 | 46.93 | 53.07 | 4,258.57 ± 251.78 | 36.96 | 63.04 |
| 0.2 mM CA | 7,082.00 ± 55.46 | 42.65 | 57.35 | 4,005.77 ± 100.93 | 34.77 | 65.23 |
| 0.2 mM PCA | 6,569.00 ± 84.55 | 39.56 | 60.44 | 2,622.47 ± 154.13 | 22.76 | 77.24 |
| 0.5 mM HA | 7,549.67 ± 59.53 | 45.47 | 54.53 | 2,988.77 ± 118.56 | 25.94 | 74.06 |
a
Dickeya zeae MS2 cells carrying the GFP reporter pPhrpA were grown in LS5 supplemented with optimal concentration of each compound for 14 h. GFP MFI of bacterial cells was determined by Microplate Reader and Flow Cytometer. Three replicates were used in each compound and three independent experiments were performed.
b
DMSO% represents the relative promoter activity of hrpA, calculated according to the formula: %DMSO = 100 × MFIcompound/MFIDMSO.