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. 2022 Mar 7;13(3):216. doi: 10.1038/s41419-022-04661-8

Fig. 2. N6AMT1-mediated 6mA modification represses proliferation and migration of BC cells in vitro.

Fig. 2

A Levels of N6AMT1 protein (top panel) and DNA 6mA levels (middle and lower panels) in various breast cancer cells were analyzed by western blot (WB, n = 3), dot-blot (n = 4), and ELISA (n = 4), respectively. β-ACTIN and methylene blue staining (MB) were used as the loading control for WB and dot-blot assays, respectively. The value is the relative ratio to BT-549 cells. Bold fonts indicate p-values less than 0.05, calculated by one-way ANOVA followed by the Bonferroni test. B Levels of N6AMT1 protein and 6mA in MDA-MB-453 cells following siN6AMT1 or si-NC transfection. The value is the relative ratio to si-NC. Bold fonts indicate p-values less than 0.05, calculated by one-way ANOVA followed by the Bonferroni test. C Levels of N6AMT1 protein and 6mA in SKBR3 cells following lentivirus-N6AMT1 (OE-N6AMT1) or -control (OE-CN) transduction. The value is the relative ratio of OE-NC. Bold fonts indicate p-values less than 0.05, calculated by Student’s t-test. D–F, Knockdown of N6AMT1 enhanced colony formation and migration of MDA-MB-453 cells, whereas N6AMT1 overexpression impaired colony formation and migration of SKBR3 cells. *p < 0.05, **p < 0.01, **p < 0.001 vs. si-CN or OE-CN.