Fig. 1. Schematic representation of Ae. albopictus Nix isoform cloning in the injected plasmids.

Isoform naming follows work published in ref. ²². Grey boxes are Nix exons, with darker grey being the translated parts. White triangles are piggyBac 5′ and 3′ inverted terminal repeat (ITR) sequences, orange boxes represent an AttP landing site included for potential future purposes, purple boxes represent loxP recombination sites, white arrows are promoters, pink polygons are SV40 polyA sequences, green, red and yellow boxes are eGFP, DsRed and YFP gene sequences, respectively. Drawing not to scale. Plasmids carry a Nix isoforms 3–4 and a GFP marker gene under the control of the OpIE2 promoter b Nix isoforms 3–4 and a GFP marker gene under the control of the polyubiquitin promoter, c Nix isoform 1 and a DsRed marker gene under the control of the polyubiquitin promoter, and d Nix isoform 2 and a YFP marker gene under the control of the polyubiquitin promoter. Detailed plasmid sequences can be found under Addgene references #173505, #173665, #173666, #173667.