Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 7;5:210. doi: 10.1038/s42003-022-03165-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — RT-qPCR results are represented by −ΔC_T, which reflects the relative expression level of each gene in a given treatment, C_T values being inversely proportional to the expression levels. AalRpS7 was used as endogenous reference gene. Grey dots represent each data point. Black dots represent the mean value of the N = 3 biological replicates, vertical lines represent 95% confidence intervals. On each panel, distinct letters represent significant difference in an ANOVA followed by a pairwise Tukey test (p-value < 0.001). a Nix relative expression. b Relative comparison of myo-sex orthologues total expression levels. c Relative expression of the candidate orthologue of the Ae. aegypti myo-fem gene, LOC109402113. d Relative expression of the candidate orthologue LOC115254984, which is annotated as a putative pseudo-gene. This primer pair could also amplify LOC115254986 due to high sequence similarity but this other pseudo-gene seems not to be expressed.