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. 2021 Oct 5;29(3):687–696. doi: 10.1038/s41418-021-00882-0

Fig. 5. WDR75 deficiency triggers the IRBC pathway and induces cellular senescence.

Fig. 5

A U2OS cells were transfected with indicated siRNAs, cultivated for 3 days and immunostained for nucleolin (NCL), fibrillarin (FIB) and p53. Bar, 10 μM. B U2OS cells were transfected with indicated siRNAs, cultivated for 3 days and cell lysates analyzed by immunoblotting with indicated antibodies. C U2OS cells were transfected with control or WDR75 siRNAs. Cell lysates were immunoprecipitated 72 h after transfection with control (IgG) or RPL5 antibodies and immunoblotted with indicated antibodies. D U2OS cells were transfected with indicated siRNAs, cultivated for 6 days and stained for senescence-associated β-galactosidase activity. Numbers indicate percentage of cells positive for senescence-associated β-galactosidase activity. Bar, 10 μM.