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. 2022 Mar 7;11:7. doi: 10.1186/s13619-021-00102-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Characterization of brain organoids derived from hiPSC on the micropillar chip. A Bright-field images of developing brain organoids at different days. Scale bar: 200 μm. B, C Immunohistochemical analysis were conducted to detect the NESTIN⁺/SOX2⁺ neural progenitor cells, TUJ1⁺differentiated neurons, PAX6⁺ forebrain, BRN2⁺ and TBR1⁺ cortical layer, vGLUT1⁺ excitatory neurons and GABA⁺ inhibitory neurons in day-38 and day-70 brain organoids. Scale bars indicated in the image