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The effect of exosomes on the differentiation of forebrain region in brain organoids. A, B Immunohistochemistry image depicting the expression of forebrain related marker PAX6 in brain organoids under exosome exposure at days 17 and 22. Scale bars: 100 μm. C, D Transcripts for forebrain specific markers FOXG1 and PAX6 were examined by qRT-PCR in brain organoids under exosome exposure at day 17 and day 22. Data are mean ± SEM. Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001
