TABLE 1.
Clinical strains of P. aeruginosa: patterns of susceptibility to fluoroquinolones and imipenem, results of complementation tests with wild-type gyrA or gyrB alleles, and DNA mutations found in gyrA, gyrB, or parC QRDR
| Strain | MIC (μg/ml) ofa:
|
Amino acid (codon) encoded by indicated gene at position:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CPX
|
SPX
|
IPM
|
gyrA
|
gyrB
|
parC
|
|||||
| NTb | gyrA+c | gyrB+d | NT | NT | 83 | 87 | 464 | 80 | 84 | |
| PAO1 | 0.12 | 0.12 | 0.12 | 0.25 | 1 | Thr (ACC) | Asp (GAC) | Ser (TCC) | Ser (TCG) | Glu (GAG) |
| 65 | 0.25 | 0.25 | 0.25 | 1 | 1 | —e | — | — | — | — |
| 21 | 2 | 0.12 | 2 | 4 | 8 | Ile (ATC) | — | — | — | — |
| 119 | 2 | 0.5 | 4 | 8 | 4 | Ile (ATC) | — | — | — | — |
| 1311 | 2 | 0.5 | 4 | 8 | 8 | Ile (ATC) | — | — | — | — |
| 44 | 4 | 0.25 | 4 | 8 | 64 | Ile (ATC) | — | — | — | — |
| 47 | 4 | 0.5 | 4 | 8 | 32 | Ile (ATC) | — | — | — | — |
| 128 | 4 | 4 | 0.06 | 16 | 2 | — | — | Phe (TTC) | — | — |
| 1273 | 4 | 0.25 | 4 | 16 | 1 | — | Gly (GGC) | — | — | — |
| 92 | 4 | 0.5 | 8 | 32 | 1 | Ile (ATC) | — | — | — | — |
| 74 | 8 | 0.12 | 4 | 32 | 2 | Ile (ATC) | — | — | — | — |
| 2120 | 8 | 4 | 0.12 | 32 | 1 | — | — | Phe (TTC) | — | — |
| P136 | 8 | 0.12 | 16 | 16 | 16 | Ile (ATC) | — | — | Leu (TTG) | — |
| 2 | 16 | 0.25 | 16 | 32 | 16 | Ile (ATC) | — | — | — | — |
| 57 | 16 | 0.25 | 16 | 32 | 16 | Ile (ATC) | — | — | — | — |
| 78 | 16 | 0.5 | 16 | 32 | 16 | Ile (ATC) | — | — | — | — |
| 134 | 16 | 0.12 | 16 | 32 | 16 | Ile (ATC) | — | — | Leu (TTG) | — |
| D | 32 | 0.25 | 16 | 32 | 32 | Ile (ATC) | — | — | Leu (TTG) | — |
| 6243 | 32 | 0.5 | 32 | 32 | 32 | Ile (ATC) | — | — | — | — |
| C6241 | 32 | 0.25 | 16 | 64 | 16 | Ile (ATC) | — | — | Leu (TTG) | — |
| J15 | 32 | 0.5 | 16 | 32 | 16 | Ile (ATC) | — | — | — | — |
| J388 | 32 | 0.5 | 32 | 32 | 32 | Ile (ATC) | — | — | — | — |
| C5904 | 32 | 0.5 | 16 | 128 | 2 | Ile (ATC) | — | — | Trp (TGG) | — |
| 76 | 32 | 0.25 | 16 | 32 | 16 | Ile (ATC) | — | — | — | — |
| 5299 | 64 | 0.12 | 16 | 64 | 32 | Ile (ATC) | — | — | — | — |
| 8 | 64 | 0.5 | 32 | 64 | 16 | Ile (ATC) | — | — | Leu (TTG) | — |
| 77 | 64 | 0.5 | 64 | 128 | 16 | Ile (ATC) | — | — | Leu (TTG) | — |
| 137 | 64 | 0.25 | 64 | 128 | 8 | Ile (ATC) | — | — | — | Lys (AAG) |
| 1746 | 64 | 0.5 | 32 | 64 | 2 | Ile (ATC) | — | — | Trp (TGG) | — |
| 2863 | 64 | 0.25 | 32 | 128 | 4 | Ile (ATC) | — | — | — | — |
| J431 | 64 | 0.5 | 32 | 32 | 32 | Ile (ATC) | — | — | — | — |
| J1375 | 128 | 0.12 | 128 | 128 | 0.5 | Ile (ATC) | — | — | Leu (TTG) | — |
a
CPX, ciprofloxacin; SPX, sparfloxacin; IPM, imipenem.
b
NT, not transformed.
c
Strain transformed with plasmid pPAW207 carrying the wild-type gyrA gene of E. coli.
d
Strain transformed with plasmid pPBW801 carrying the wild-type gyrB gene of E. coli.
e
—, amino acid and codon identical to those of strain PAO1.