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. 2022 Feb 22;10:805996. doi: 10.3389/fbioe.2022.805996

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Copyright © 2022 An, Cao, Zhang, Zhang, Zhou, Hu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plasmid DNA protection assay. (A) Same amount (1 μg) of pDNA treated by DNase I was run on a 1% agarose gel to determine the optimal DNase I concentration for DNA degradation. (B) Agarose gel electrophoresis of DNA, PDMAEMA + DNA (N/P ratio of 4.38:1), and PEI + DNA (N/P ratio of 2.6) incubated with DNase I to evaluate DNA protection against nuclease degradation.