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. 2022 Feb 22;10:805996. doi: 10.3389/fbioe.2022.805996

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Copyright © 2022 An, Cao, Zhang, Zhang, Zhou, Hu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Toxicity assay. (A) qPCR analysis of Nbrboh B in Nicotiana benthamiana leaves with different infiltrations (MgCl₂/MES (10 mM) as control, PDMAEMA + DNA (N/P ratio of 15:1), PEI + DNA (N/P ratio of 15:1), and 1% SDS at a time course. (B) Photosystem II Fv/Fm of different locations treated with PDMAEMA + DNA (N/P ratio of 15:1), PEI + DNA (N/P ratio of 15:1), 1% SDS, and MgCl₂/MES (10 mM) as control on the same Nicotiana benthamiana leaf. (C) Statistical data of the (B) Fv/Fm ratio represent the variable/maximum fluorescence measurement of the photosystem II quantum efficiency. (D) Phenotype of the single Nicotiana benthamiana leaf infiltrated with MgCl₂/MES (10 mM), PDMAEMA + DNA (N/P ratio of 15:1), PEI + DNA (N/P ratio of 15:1), and 1% SDS on different positions. (E) Phenotype of a single Nicotiana benthamiana leaf infiltrated with MgCl₂/MES (10 mM), 1% SDS, PDMAEMA (10 mg/mL), and PEI (10 mg/mL) on different positions.