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. 2022 Feb 22;10:805996. doi: 10.3389/fbioe.2022.805996

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Copyright © 2022 An, Cao, Zhang, Zhang, Zhou, Hu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SCHEME 1 — PDMAEMA + FAM-siRNA_NPR1-infiltrating Nicotiana benthamiana leaves. FAM-siRNA_NPR1 (20 μM) was mixed with PDMAEMA (1 mg/mL) at the fixed N/P ratio of 15 and incubated at room temperature for 30 min. The formed complexes were diluted 10 times in 10 mM MgCl₂/MES (pH 5.7) before infiltration, taking only infiltrating PDMAEMA or FAM-siRNA_NPR1 as a control.