TABLE 5.
Cytotoxic activity of spleen cells obtained from rats immunized with allogeneic E4 tumor cells followed by CAM
| Treatment of spleen cell donor | % Cytolysis at effector/target cell ratio
|
|||
|---|---|---|---|---|
| Day 12
|
Day 15
|
|||
| 1:50 | 1:25 | 1:50 | 1:25 | |
| Immunization alone | 5.3 ± 0.9 | 3.9 ± 2.1 | 5.1 ± 0.0 | 3.2 ± 1.0 |
| CAM alone | 3.3 ± 1.0 | 1.6 ± 0.7 | 6.9 ± 2.0 | 2.0 ± 1.9 |
| Immunization + CAM | 20.0 ± 1.8b | 15.9 ± 0.6b | 18.0 ± 1.9b | 13.4 ± 1.5b |
| None | 5.4 ± 0.7 | 2.0 ± 1.6 | 6.1 ± 1.6 | 2.0 ± 0.8 |
a
F-344 rats were immunized with viable E4 tumor cells on day 3 (3 × 107 cells s.c.) and day 9 (3 × 107 i.p.). CAM was administered at 2 mg/kg p.o. on days 1 to 14. Spleens in each group were obtained from three rats. The 51Cr release assay was performed on days 12 and 15.
b
P < 0.001 versus the immunization-alone group.