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. 2021 Oct 29;35(4):596–604. doi: 10.5713/ab.21.0333

Table 3.

Reaction system for first polymerase chain reaction

Ingredients Volume
2×Hieff Robust PCR Master Mix 15 μL
Bar-PCR primer F 1 μL
Primer R 1 μL
PCR product 10 to 20 ng
H2O 9 to 12 μL
Total volume 30 μL

PCR, polymerase chain reaction.