Table 5.
Reaction system for second polymerase chain reaction
| Ingredients | Volume |
|---|---|
| 2×Hieff Robust PCR Master Mix | 15 μL |
| Primer F | 1 μL |
| Index-PCR Primer R | 1 μL |
| PCR products | 20 to 30 ng |
| H2O | 9 to 12 μL |
| Total volume | 30 μL |
PCR, polymerase chain reaction.
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