Table 5.

Immune responses and serum protein fractions in commercial broilers fed different methyl donors in diet containing no supplemental methionine

Treatment	Immune responses		Serum
	LPR	ND titre, log 2	Protein (g/dL)	Albumin (g/dL)
CD	0.635A	4.50	2.577B	0.522ABC
BD	0.393CD	4.33	1.737C	0.279D
FA (4 mg/kg)	0.433C	4.50	3.800A	0.693A
Biotin (1.5 mg/kg)	0.350D	4.00	3.072B	0.465BCD
Vit. B12 (0.1 mg/kg)	0.453BC	4.00	1.238C	0.323CD
Betaine, 0.2%	0.497B	4.50	2.993B	0.592AB
N	10	10	10	10
P	0.001	0.863	0.001	0.002
SEM	0.0186	0.1288	0.1542	0.0320

LPR, lymphocyte proliferation ratio; ND, Newcastle disease; CD, control diet; BD, basal diet; FA, folic acid; P probability; SEM standard error mean.

^A–DMeans having no common super script in a column differ significantly (p<0.05).