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. 2022 Feb 22;12:808480. doi: 10.3389/fphar.2021.808480

FIGURE 3.

FIGURE 3

Fisetin attenuates DOX-induced ferroptosis. (A) Western blot results of GPX4 levels in rat heart tissues. GAPDH was used as normalization control. (B) Quantification of the GPX4 level from Western blot (A). (C–D) Western blot results of GPX4 levels in H9c2 cells; the expression was quantified by ImageJ analysis. (E) Results of the CCK-8 assay. H9c2 cells exposed to different concentrations of DOX for 24 h. (F) Cell survival rate of H9c2 cells exposed to 1 µM DOX using CCK-8 assay. (G) Cell survival rate of co-administration of DOX + fisetin (20 and 40 µM) in H9C2 cells. (H–K) MDA, GSH, ROS, and iron concentration levels in cells in different groups. (L–O) MDA, GSH, ROS, and iron concentration levels in heart tissues. The values are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; NS, no significance; GPX4, glutathione peroxidase 4; GSH, glutathione; ROS, reactive oxygen species; MDA, malondialdehyde; CCK-8, Cell Counting Kit-8.