FIGURE 5.

Evaluation of p44/42 MAPK involvement in AdipoR plus Gem effects. MIA PaCa-2 (A) and PANC-1 (B) were treated and not (control) with 10 µg/ml AdipoR, 15 nM (MIA PaCa-2) or 50 nM (PANC-1) Gem, and AdipoR plus Gem over a period of 48 h. Thereafter, either single or combination consequences on p44/42 MAPK activation (phosphorylation) were estimated by Western blotting. Phospho-MAPK/MAPK ratio results from the quotient of phospho-p44/42 MAPK and its relative housekeeping on the gel divided by a quotient of p44/42 and its relative α-tubulin. (C) MIA PaCa-2 was exposed and not (control) to 10 μM PD98059 for 24 h, and the cell growth percentage was established. (D) MIA PaCa-2 single and combination treatments in MAPK-proficient and -hampered background. (E) Combination treatments containing 10 µg/ml AdipoR plus 50 nM Gem were carried out in PANC-1 cells with or without PD98059 inhibitor. Two hours of PD98059 pretreatment preceded and not individual and combinatory administration. Results are depicted in percentage as mean ± SD of three independent experiments. *p < 0.05 by Tukey’s multiple comparisons or Student’s t-test.