FIGURE 6.

Responsiveness of MIA PaCa-2 Gem-resistant cells to single and combinatory treatments. Either MIA PaCa-2 Gem-sensitive and -resistant cells were treated and not (control) with 10 µg/ml AdipoR, 15 nM Gem, and AdipoR plus Gem for 24 (A) and 48 h (B); thereafter, the relative impact on cell growth was addressed. (C) Cell media of both MIA PaCa-2 Gem-sensitive and -resistant cells were supplemented with and without (control) 10 nM Gem for 8 days. Illustrative violet-stained wells are shown on the left side, the relative quantification on the right. (D) MIA PaCa-2 Gem-resistant cells undergoing AdipoR (10 µg/ml) and Gem (5 nM) individually and combinatory treatments were tested for colony-forming ability. Images and quantification assay are provided in Figure. (E) MIA PaCa-2 Gem-sensitive and -resistant were incubated either with single or combination drugs as indicated in (A). FACSCelesta^TM analysis was later performed with the purpose of defining the drug-induced consequences on cell phase distribution. Reported results are indicated in percentage as median value ± SD of triplicate experiments. *p < 0.05, **p < 0.01, ***p < 0.001 by Tukey’s multiple comparisons or Student’s t-test.