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. 2022 Feb 22;13:783879. doi: 10.3389/fmicb.2022.783879

FIGURE 5.

FIGURE 5

Effect of digestive juice treatment on KT-11 LE and KT-11 SLP. SDS-PAGE patterns of (A) KT-11 LE and fractionated LE, (B) KT-11 SLP after treatment with simulated gastric juice (1.6% pepsin in 0.1 M KCl/HCl, pH 2.0) and intestinal juice (0.1% pancreatin in PBS, pH 7.2) for the indicated time periods. Lane 1 (M1) and lane 7 (M2): molecular weight marker, lanes 2–6: digestion pattern by gastric juice for the indicated time periods (h), lanes 8–14: digestion pattern by intestinal juice for the indicated time periods (h). (C) Inhibitory effect on DS-1 infection in pre-infection treatment with KT-11 SLP digested with gastric juice for the indicated times periods. The results are shown as means with standard deviations relative to KT-11 SLP-untreated viral-infected control (n = 3). PC represents the condition of viral-infected and KT-11 SLP-untreated condition. Different letters denote a significant difference at p < 0.01.