Table 1.
Primer sequences and details of the candidate genes used in quantitative real-time PCR (qRT-PCR).
| Gene name | Accession no. | Sequence (5–3′) | Amplicon size (bp) | Primer efficiency (%) | Regression coefficient (R2) | Linear regression |
|---|---|---|---|---|---|---|
| GAPDH | KT361883.1 | F:CAAGATGGCTTCCTCGTA | 161 | 96.74 | 0.9996 | y = 3.4025x + 14.823 |
| R:GCACCACCCTCTAAATGA | ||||||
| β-ACT | MT880275.1 | F:GTATGGAATCTTGCGGTATC | 76 | 92.97 | 0.9996 | y = 3.5028x + 13.025 |
| R:AGGTCCTTACGGATGTCA | ||||||
| RPL32 | MT883789.1 | F:CCATCAATCGGATCGCTAT | 178 | 95.74 | 0.9958 | y = 3.4283x + 15.441 |
| R:ATTGTGGACCAGGACCTT | ||||||
| RPL40 | MT883790.1 | F:CAAGCGAAACTGGCGTAA | 96 | 91.43 | 0.9989 | y = 3.5461x + 14.565 |
| R:TTTGAACCGTAACCGATG | ||||||
| EF1-α | MT883788.1 | F:GCTGATTGTGGGAGTGAA | 144 | 90.37 | 0.9985 | y = 3.5767x + 13.088 |
| R:CCAGAAATGGGTACGAAA | ||||||
| α-TUB | MT880274.1 | F:GACTCCTTCAACACCTTCTT | 98 | 90.04 | 0.9981 | y = 3.5863x + 13.786 |
| R:CGGACCTCATCAACTACAG | ||||||
| β-TUB | MT883787.1 | F:CTCAACATCCAGAACAAGAAC | 168 | 88.65 | 0.9971 | y = −3.6278x + 13.901 |
| R:GGTGAACTGCTCCGAGAT | ||||||
| 18S | MT889641.1 | F:TGTCTGCCTTATCAACTTTC | 113 | 89.32 | 0.9999 | y = −3.6076x + 3.4139 |
| R:GATGTGGTAGCCGTTTCTCA | ||||||
| 28S | MT889642.1 | F:CCCGACGCAAGTCAACG | 96 | 85.02 | 0.9999 | y = −3.7423x + 10.850 |
| R:GCACAGTCCGAGACAGCAC | ||||||
| AK | MT880273.1 | F:CGATGACCACTTCCTGTT | 153 | 86.28 | 0.9970 | y = 3.7014x + 14.800 |
| R:ATGGAGATAAGACGGAGATG | ||||||
| CSP1 | KT357395.1 | F:TCTGCTGGTGCCCTATAT | 183 | 104.16 | 0.9940 | y = −3.2262x + 21.469 |
| R:TTCGTGGTTGATGAGATGG | ||||||
| SOD | MT883791.1 | F:ATTGAGGCGGATGTTGCT | 151 | 85.41 | 0.9991 | y = −3.7295x + 17.319 |
| R:TCTTGCTGAGGTTGTGGC |