Table 2.

Expression stability of the candidate reference genes in A. dissimilis under different biotic conditions.

Biotic condition	Reference gene	geNorm		Normfinder		Bestkeeper		ΔCt
		Stability	Rank	Stability	Rank	Stability	Rank	Stability	Rank
Developmental stages	18S	0.66	5	0.32	3	0.74	8	0.52	3
	28S	0.73	6	0.53	5	0.50	3	0.71	5
	AK	0.95	7	0.81	8	0.79	9	1.54	8
	EF1-α	0.44	1	0.31	1	0.64	7	0.51	2
	GAPDH	1.25	9	1.07	9	0.48	2	1.58	9
	RPL32	0.58	3	0.59	6	0.33	1	0.76	6
	RPL40	0.44	1	0.32	2	0.56	5	0.29	1
	α-TUB	0.49	2	0.42	4	0.62	6	0.71	4
	β-ACT	1.12	8	1.08	10	0.84	10	1.93	10
	β-TUB	0.61	4	0.64	7	0.54	4	1.00	7
Larval tissues	18S	0.58	4	0.54	6	0.07	2	0.39	1
	28S	0.80	7	0.77	8	0.05	1	0.78	7
	AK	1.17	8	1.52	9	0.79	9	2.51	9
	EF1-α	0.40	2	0.51	5	0.16	4	0.52	5
	GAPDH	0.66	5	0.24	2	0.75	8	0.72	6
	RPL32	0.37	1	0.54	7	0.16	3	0.43	4
	RPL40	0.37	1	0.21	1	0.59	7	0.40	2
	α-TUB	0.47	3	0.38	3	0.29	5	0.42	3
	β-ACT	1.50	9	1.83	10	0.97	10	3.02	10
	β-TUB	0.72	6	0.44	4	0.55	6	0.92	8
Adult tissues	18S	0.41	2	0.30	3	0.97	7	1.74	3
	28S	0.35	1	0.29	2	0.98	10	1.34	1
	AK	1.05	8	0.90	9	0.86	3	2.31	10
	EF1-α	0.66	4	0.49	5	0.91	5	1.75	5
	GAPDH	0.90	7	0.67	8	0.85	2	1.74	4
	RPL32	0.70	5	0.46	4	0.97	8	2.19	8
	RPL40	0.78	6	0.66	7	0.93	6	2.26	9
	α-TUB	0.35	1	0.19	1	0.98	9	1.52	2
	β-ACT	1.18	9	1.06	10	0.70	1	1.92	7
	β-TUB	0.58	3	0.61	6	0.90	4	1.90	6