Table 4.
Expression stability of the candidate reference genes in A. dissimilis under different abiotic conditions.
Abiotic condition | Reference gene | geNorm | Normfinder | Bestkeeper | ΔCt | ||||
---|---|---|---|---|---|---|---|---|---|
Stability | Rank | Stability | Rank | Stability | Rank | Stability | Rank | ||
Diets | 18S | 0.07 | 1 | 0.13 | 8 | 0.77 | 3 | 0.07 | 1 |
28S | 0.09 | 2 | 0.16 | 9 | 0.66 | 1 | 0.12 | 3 | |
AK | 0.13 | 3 | 0.06 | 3 | 0.92 | 9 | 0.16 | 5 | |
EF1-α | 0.16 | 5 | 0.03 | 2 | 0.99 | 10 | 0.19 | 7 | |
GAPDH | 0.31 | 9 | 0.51 | 10 | 0.76 | 2 | 0.80 | 10 | |
RPL32 | 0.07 | 1 | 0.13 | 7 | 0.80 | 5 | 0.12 | 2 | |
RPL40 | 0.14 | 4 | 0.01 | 1 | 0.88 | 8 | 0.12 | 4 | |
α-TUB | 0.20 | 8 | 0.13 | 6 | 0.87 | 7 | 0.21 | 8 | |
β-ACT | 0.17 | 6 | 0.09 | 4 | 0.79 | 4 | 0.26 | 9 | |
β-TUB | 0.19 | 7 | 0.12 | 5 | 0.85 | 6 | 0.18 | 6 | |
Insecticide | 18S | 0.37 | 3 | 0.14 | 2 | 0.82 | 7 | 0.26 | 2 |
28S | 0.46 | 6 | 0.34 | 7 | 0.15 | 1 | 0.27 | 3 | |
AK | 0.69 | 9 | 0.74 | 10 | 0.32 | 2 | 1.04 | 10 | |
EF1-α | 0.50 | 7 | 0.47 | 8 | 0.84 | 8 | 0.86 | 9 | |
GAPDH | 0.59 | 8 | 0.48 | 9 | 0.38 | 3 | 0.68 | 8 | |
RPL32 | 0.43 | 5 | 0.24 | 4 | 0.46 | 4 | 0.25 | 1 | |
RPL40 | 0.39 | 4 | 0.11 | 1 | 0.72 | 5 | 0.31 | 4 | |
α-TUB | 0.21 | 1 | 0.20 | 3 | 0.91 | 10 | 0.57 | 5 | |
β-ACT | 0.28 | 2 | 0.28 | 5 | 0.82 | 6 | 0.62 | 6 | |
β-TUB | 0.21 | 1 | 0.31 | 6 | 0.88 | 9 | 0.67 | 7 | |
Temperature | 18S | 0.13 | 1 | 0.12 | 3 | 0.89 | 10 | 0.30 | 6 |
28S | 0.20 | 3 | 0.03 | 1 | 0.79 | 7 | 0.24 | 3 | |
AK | 0.45 | 8 | 0.40 | 8 | 0.46 | 4 | 0.60 | 9 | |
EF1-α | 0.13 | 1 | 0.15 | 6 | 0.83 | 8 | 0.28 | 5 | |
GAPDH | 0.51 | 9 | 0.45 | 10 | 0.28 | 2 | 0.63 | 10 | |
RPL32 | 0.33 | 6 | 0.35 | 7 | −0.05 | 1 | 0.41 | 7 | |
RPL40 | 0.23 | 4 | 0.13 | 4 | 0.42 | 3 | 0.18 | 1 | |
α-TUB | 0.25 | 5 | 0.14 | 5 | 0.68 | 6 | 0.28 | 4 | |
β-ACT | 0.39 | 7 | 0.44 | 9 | 0.46 | 5 | 0.58 | 8 | |
β-TUB | 0.16 | 2 | 0.04 | 2 | 0.89 | 9 | 0.22 | 2 | |
Starvation | 18S | 0.08 | 3 | 0.03 | 3 | 0.84 | 5 | 0.11 | 5 |
28S | 0.14 | 6 | 0.03 | 2 | 0.91 | 7 | 0.23 | 7 | |
AK | 0.11 | 5 | 0.24 | 8 | −0.16 | 1 | 0.11 | 4 | |
EF1-α | 0.09 | 4 | 0.01 | 1 | 0.94 | 9 | 0.14 | 6 | |
GAPDH | 0.03 | 1 | 0.16 | 7 | 0.35 | 2 | 0.03 | 2 | |
RPL32 | 0.33 | 9 | 0.54 | 10 | 0.92 | 8 | 0.91 | 10 | |
RPL40 | 0.22 | 8 | 0.19 | 8 | 0.86 | 6 | 0.47 | 9 | |
α-TUB | 0.06 | 2 | 0.09 | 5 | 0.83 | 4 | 0.06 | 3 | |
β-ACT | 0.03 | 1 | 0.14 | 6 | 0.38 | 3 | 0.01 | 1 | |
β-TUB | 0.17 | 7 | 0.04 | 4 | 0.97 | 10 | 0.32 | 8 |