Table 5.
Expression stability of the candidate reference genes in A. dissimilis under all samples.
| Reference gene | geNorm | Normfinder | Bestkeeper | ΔCt | ||||
|---|---|---|---|---|---|---|---|---|
| Stability | Rank | Stability | Rank | Stability | Rank | Stability | Rank | |
| 18S | 0.37 | 1 | 0.30 | 1 | 0.85 | 10 | 0.75 | 2 |
| 28S | 0.37 | 1 | 0.43 | 4 | 0.71 | 4 | 0.70 | 1 |
| AK | 1.08 | 8 | 0.96 | 9 | 0.61 | 2 | 1.62 | 9 |
| EF1-α | 0.62 | 3 | 0.44 | 5 | 0.78 | 8 | 0.98 | 4 |
| GAPDH | 0.91 | 7 | 0.79 | 8 | 0.57 | 1 | 1.11 | 8 |
| RPL32 | 0.77 | 6 | 0.52 | 7 | 0.75 | 5 | 1.09 | 7 |
| RPL40 | 0.72 | 5 | 0.42 | 3 | 0.79 | 9 | 1.05 | 6 |
| α-TUB | 0.58 | 2 | 0.38 | 2 | 0.77 | 7 | 0.86 | 3 |
| β-ACT | 1.23 | 9 | 1.15 | 10 | 0.66 | 3 | 2.00 | 10 |
| β-TUB | 0.67 | 4 | 0.51 | 6 | 0.76 | 6 | 0.97 | 5 |