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. 2021 Dec 7;30(2):162–169. doi: 10.4062/biomolther.2021.062

Fig. 5.

Fig. 5

Effect of Fas21 on LSECs’ chemotactic migration induced by B16M cells and by tumor-activated HSCs: (A) HSCs were cultured on collagen-coated inserts in the presence of increasing concentrations of Fas21. Then, media was replaced by DMEM in the cell compartment and supernatants from B16M in the lower compartment. Migrated cells were stained with H/E and counted under the microscope. *p<0.05 versus control cells in basal medium number of migrated HSCs in basal medium: 15 ± 8 and #p<0.05 versus control cells in B16M-CM. (B) LSECs were cultured on collagen-coated inserts and either 10 μM of Fas21 or vehicle. Then, media was changed and replaced by basal media in the upper cell compartment and supernatants from B16M-treated HSCs in the lower compartment. Migrated cells were stained with H/E and counted under the microscope. *p<0.05, **p<0.01 and ***p<0.005 versus control LSECs in basal medium (10 ± 2 LSECs) and #p<0.05 versus control cells in B16M-CM. F21: Fas21.