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. 2021 Jul 29;30(2):137–144. doi: 10.4062/biomolther.2021.088

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Copyright © 2022, The Korean Society of Applied Pharmacology

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Shikonin induces apoptosis in SNU-C5RR colon cancer cells. (A) Apoptotic bodies (arrows) were observed via fluorescence microscopy in SNU-C5RR cells stained with Hoechst 33342 and quantified. *p<0.05 vs the control group. (B) Apoptosis in SNU-C5RR cells was examined via the TUNEL assay after 24 h of 38 μM shikonin treatment. TUNEL-positive cells are indicated by arrows. *p<0.05 vs the control group. (C) DNA damage was assessed using an alkaline comet assay. Representative images and the percentage of total DNA fluorescence in the comet tails are shown. *p<0.05 vs the control group.