Skip to main content
. 2022 Feb 22;13:825032. doi: 10.3389/fimmu.2022.825032

Figure 2.

Figure 2

GPR15L expression in keratinocytes and GPR15L-mediated control for epidermal differentiation. (A) Quantitative RT-PCR analysis of mRNA levels of keratinocyte differentiation markers and GPR15L in mouse primary cultured keratinocytes during Ca2+-induced differentiation. Results were normalized to Gapdh expression (error bars, SD; n = 3 per group). (B) Quantitative RT-PCR analysis of mRNA levels of keratinocyte differentiation markers in primary cultured keratinocytes from wild-type or GPR15L-null mice on day three of Ca2+-induced differentiation. Results were normalized to Gapdh expression and indicated as expression levels relative to those of the wild-type (error bars, SD; n ≥8 per group). (C) Quantitative RT-PCR analysis of mRNA levels of keratinocyte differentiation markers in 3D-cultured human epidermis treated with GPR15L during development in vitro. Results were normalized to GAPDH expression (error bars, SD; n = 3 per group). (D) Protein expression levels of keratinocyte differentiation markers in 3D-cultured human epidermis treated with or without 10 µM GPR15L during development in vitro. Representative results from triplicates are shown (a scale bar, 100 µm). (E) Thickness of the filaggrin-positive and loricrin-positive layers in the 3D-cultured human epidermis treated with or without GPR15L during development in vitro. Thickness of filaggrin or loricrin-positive layers of three sections from each of the triplicates were measured at three or more sites (error bars, SD; n ≥ 30 per group). (F) Quantitative RT-PCR analysis of GPR15L mRNA levels in mouse primary cultured keratinocytes with indicated stimulations. Results were normalized to Gapdh expression (error bars, SD; n = 3 per group).