Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 22;10:815067. doi: 10.3389/fcell.2022.815067

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Luo, Ma, Zheng, Su and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Basic application of colorectal organoids in disease and basic research. Organoids can be established from healthy or CRC tissue from the patient-sourced samples. Isolated tissue or stem cells, or activin-treated human pluripotent stem cells, are embedded in a basement membrane matrix and maintained in media containing a variety of niche factors critical for expansion. Organoids are usually formed in 3D and have structures similar to their origin. For example, culturing isolated gastric epithelial cells under the conditions described above can grow human gastric organoids in which gastric gland zone buds are distributed around the central lumen. Gene editing of organoids can be used for the modeling of diseases as well as the construction of xenografts. Direct sampling through mice and organoids can be used for a series of fundamental studies.