TABLE 1.
Summary of the different methods related to CRC organoids of mouse and human.
| References | Medium | Tissues |
|---|---|---|
| Sato Sato et al. (2009) | AdvDMEM/F12, 10–50 ng/ml EGF,500 ng/ml R-spondin-1,100 ng/ml Noggin | Primary mouse tissue |
| Schatoff Schatoff et al. (2019) | AdvDMEM/F12, penicillin/streptomycin, Glutamine, 1 mM N-Acetylcysteine, 10 U/ml DNAseⅠ, 1 ml FBS(final 5%) | Primary mouse tissue |
| Goto Goto et al. (2019) | AdvDMEM/F12, 50 ng/ml mouse EGF, penicillin/streptomycin, 10 mM HEPES, GlutaMAX, B27(1×) | Mouse |
| CRC | ||
| Ganzleben Ganzleben et al. (2020) | AdvDMEM/F12, 10 nM HEPES, 2 mM GlutaMAX, Pen/Strep Amphotericin Mix, B27(1×), 1 mM Acetylcysteine, 50 ng/ml EGF | Mouse |
| CRC | ||
| Sato Sato et al. (2011) | AdvDMEM/F12, penicillin/streptomycin, 10 mM HEPES, Glutamax, N2(1×), B27(1×), 1 mM N-acetylcysteine, murine EGF, murine noggin, human R-spondin-1, human Want-3A, gastrin, nicotinamide, A83-01, SB202190, Y-27632 | Human |
| CRC | ||
| Van de Wetering van de Wetering et al. (2015) | AdvDMEM/F12, 1% penicillin/streptomycin, 1% Hepes buffer, 1% Glutamax, 20% R-spondin-1 conditioned medium, 10% Noggin conditioned medium, B27(1×), 1.25 mM n-Acetyl Cysteine, 10 mM Nicotinamide, 50 ng/ml EGF, 500 nM A83-01, 10 μM SB202190, 100 μg/ml Primocin, 10 μM Y-27632 | Human |
| CRC | ||
| Fujii Fujii et al. (2016) | AdvDMEM/F12, penicillin/streptomycin, 10 mM HEPES, 2 mM GlutaMAX, B27(1×), 10 nM gastrin I, 1 mM N-acetylcysteine, 50 ng/ml mouse recombinant EGF, 100 ng/ml mouse recombinant Noggin, 10% R-Spondin-1 conditioned medium, 50% Wnt-3A conditioned medium, 500 nM A83-01, 10 μM SB202190 | Human |
| CRC | ||
| Buzzelli Buzzelli et al. (2018) | DMEM/F12, GlutaMAX, 1% StemPro, 10 ng/ml Y-27632, 100 ng/ml R-Spondin-1, 10 ng/ml Noggin, 10 ng/ml WNT3A, 10 ng/ml EGF, 5 ng/ml IGF-1, 10 ng/ml FGF-10, 10 ng/ml FGFβ, 10 ng/ml ET3 | Human CRC liver metastases pathological specimens |
| Toden Toden et al. (2018) | AdvDMEM/F12, 50%(v/v) L-WRN conditioned medium, 20% FBS, 2 mM L-glutamine, 0.2% Primocen, 10 μM Y-27632, 10 μM SB431542, 5% penicillin/streptomycin | APCMin mouse CRC and human CRC |
| Ubink Ubink et al. (2019) | AdvDMEM/F12, penicillin(100 U/ml), streptomycin(100 μg/ml), 10 mM Hepes, 400 μM Glutamax, B27(1×), 1 mM N-Acetyl-L-cysteine, 50 ng/ml Noggin, 500 nM A83-01, 10 μM SB202190 | Human CRC peritoneal metastases pathological specimens |
| Ng Ng et al. (2019) | AdvDMEM/F12, penicillin/streptomycin, 10 mM HEPES, 2 mM Glutamax, B27(1×), 1 mM N-acetylcysteine, 10 nM gastrinⅠ, 50 ng/ml recombinant human noggin, 50 μg/ml recombinant human R-spondin-1, 500 nM A83-01, 10 mM SB202190, 10 μM Y-27632 | Human |
| CRC | ||
| Mukohyama Mukohyama et al. (2019) | AdvDMEM/F12, 2 mM GlutaMAX, 10 mM HEPES, 1 mM sodium pyruvate, 10% heat-inactivated FBS, 120 μg/ml penicillin, 100 μg/ml streptomycin, 0.25 μg/ml amphotericin-B, ITES mdia supplement, 50 ng/ml hEGF, 500 ng/ml hR-Spondin-1, 100 ng/ml hNoggin, 10 μM Y-27632 | Cell lines and human CRC PDX |
| Lee Lee et al. (2019) | AdvDMEM/F12, B27(1×), 1.25 mM N-acetyl cysteine, 50 ng/ml human EGF, 50 ng/ml human Noggin, 10 nM gastrin, 500 nM A83-01, 100 mg/ml primocin, 10 μM Y-27632 | Human |
| CRC | ||
| Knight Knight et al. (2021) | AdvDMEM/F12, HEPES(5 mM), 2 mM L-glutamine, 100 U/ml penicillin/streptomycin, N2(1×), B27(1×), 100 ng/ml noggin and 50 ng/ml EGF, 500 ng/ml R-spondin, 10 nM gastrin, 100ng/ml Wnt-3A, 10 μM Y-27632, 0.5 μM A83-01, 5 μM SB202190, 4 mM nicotinamide, 10 ng/ml FGF basic, 10 ng/ml FGF10, 1 μM prostaglandin E2 | Human |
| CRC | ||
| Costales-Carrera Costales-Carrera et al. (2020) | AdvDMEM/F12, Hepes 10 mM, Glutamax 10 mM, N2(1×), B27(1×), 1 mM N-acetyl-L-cysteine, Primocin(1:500), 0.1 μg/ml Noggin, 1 μg/ml Gastrin, 10 μM Y-27632, 50 ng/ml EGF | Human |
| CRC | ||
| Narasimhan Narasimhan et al. (2020) | AdvDMEM/F12, 10 mM Hepes, Glutamax(1×), 10 mg/L gentamicin, antibiotic-antimycotic(1×), B27(2×), 500 nM A83-01, 50 ng/ml hEGF, 1 nM Gastrin 1 human, 1 mM M-Acetyl-L-cysteine, 5 μM SB202190, 10 μM SB431542, 10 μM Y27632 | Human |
| CRC | ||
| Song Song et al. (2020) | AdvDMEM/F12, 50%(v/v)L-WRN-conditioned medium, Wnt3a, R-spondin, Noggin, penicillin/streptomycin(1×), 10 mM HEPES, 2 mM GlutaMAX, B27(1×), N2(1×), 1 mM N-Acetylcysteine, 10 nM Gastrin, 10 mM nicotinamide, 10 μM SB202190, 50 ng/ml recombinant murine EGF, 0.5 μM A83-01 | Human |
| CRC | ||
| Costales-Carrera Costales-Carrera et al. (2020) | AdvDMEM/F12, 10 mM HEPES, 10 mM Glutamax, N2(1×), B27(1×), 1 mM N-acetyl-L-cysteine, Primocin(1:500), 0.1 μg/ml Noggin, 1 μg/ml Gastrin, 10 μg/ml, 10 μM Y-27632, 50 ng/ml EGF, 0.02 μM PGE2, 1 μM LY-2157299, 10 μM SB-202190 | Human |
| CRC |