Skip to main content
. 2022 Feb 15;14(2):899–908.

Figure 5.

Figure 5

Let-7c induces BACE2 expression through RNAa. A. Schematic diagram of the structure of BACE2 promoter and three deleted constructs; B. pB2Luc-A, pB2Luc-B, pB2Luc-C and pB2Luc-D transfected into HEK293 cells with pmiR-let-7c or not, respectively, and dual luciferase assay performed 48 hours after transfection. *P<0.05, compared to the pGL3-Basic group; C. Sequence alignment of BACE2 and let-7c, with sequences of BACE2 and let-7c aligned using ClustalW2 software; D. HEK293 cells transfected with pshAGO1 or pshAGO2, and AGO1 or AGO2 expression detected by RT-PCR. *P<0.05, compared to the control group; E. HEK293 cells transfected with pshAGO1 or pshAGO2, and BACE2 expression detected by RT-PCR. *P<0.05, compared to the si-control group; F. pAPP-C99Flag and pmiR-let-7c transfected into HEK293 cells with pshAGO1 or pshAGO2, and C99 protein detected by M2 antibody. *P<0.05, compared to the si-control group. Values represent means ± SD, n=3.