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. 2022 Feb 15;14(2):1188–1203.

Figure 1.

Figure 1

Cannabidiol suppresses osteosarcoma cell proliferation and migration. A, B. Different concentrations of cannabidiol (0, 2.5, 5, 10, 15, 20 µm) were added to MG63 and HOS cells and cultured for 48 h. Cell viability was measured by the MTT assay. **P<0.01 vs. Vehicle; n=6. C, D. Representative osteosarcoma cell colony formation images and statistical analysis of cell colony formation rate after adding different concentrations of cannabidiol. **P<0.01 vs. Vehicle; n=3. E, F. Wound closure of MG63 and HOS cells cultured with or without cannabidiol (10 µM) at 0 h and 24 h. Wound width = wound area/wound height in each group. Scale bar: 100 µm. *P<0.05, **P<0.01 vs. Vehicle; n=5. All results are presented as mean ± SEM.