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. 2022 Feb 15;14(2):831–848.

Figure 6.

Figure 6

TRIM24 overexpression recruited macrophages to tumors. A. CD206 staining in MC38-V and MC38-TRIM24 tumors was analyzed by IHC. Scale bar: 50 μm. B. mRNA expression of indicated genes was determined by RT-qPCR in MC38-V and MC38-TRIM24 cells. C. mRNA expression of CCL2, CCL5 and CSF-1 in HCT-8-V and HCT-8-TRIM24 cells. D. Protein level of VEGF, CCL2, CCL5 and CSF-1 in HCT-8-V and HCT-8-TRIM24 cells was analyzed by western blotting and normalized to β-actin. E. mRNA expression of VEGF, CSF-1, CCL2 and CCL5 was determined by RT-qPCR in LS180 con-KD and TRIM24-KD cells. F. Protein level of CCL2, CCL5 and CSF-1 in LS180 con-KD and TRIM24-KD cells was analyzed by western blotting and normalized to β-actin. G. mRNA expression of VEGF, CSF-1, CCL2 and CCL5 was determined by RT-qPCR in HCT-8 con-KD and TRIM24-KD cells. H. Protein level of CCL2, CCL5 and CSF-1 in HCT-8 con-KD and TRIM24-KD cells was analyzed by western blotting and normalized to β-actin. Data are presented as the mean ± SD from three independent experiments. *P<0.05; **P<0.01.