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. 2022 Feb 4;298(3):101686. doi: 10.1016/j.jbc.2022.101686

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Generation and characterization of Sdccag8^ΔC/ΔCmice.A, DNA sequences encoding around the Sdccag8 C-terminal region of Sdccag8^+/+ and Sdccag8^ΔC/ΔC mice. The Arg537Stop codon and EcoRI restriction site were underlined in red. The Arg537Stop codon and EcoRI restriction site were knocked-in before Sdccag8-C (537–717 aa) in Sdccag8^ΔC/ΔC mice. B, PCR analysis of Sdccag8 from tail tips of Sdccag8^+/+, Sdccag8^ΔC/+, and Sdccag8^ΔC/ΔC mice. The PCR products from Sdccag8^ΔC/+ and Sdccag8^ΔC/ΔC mice were able to be digested by EcoRI. C, RT-PCR analysis of Sdccag8 (Exons 11–13) and β-actin in MEFs from Sdccag8^+/+ and Sdccag8^ΔC/ΔC mice. β-actin was used as a loading control. Sdccag8 mRNA was detected in Sdccag8^ΔC/ΔC mice. D, genotype distribution of the offspring from Sdccag8^ΔC/+ parents at P0-2 and P12-1 month old. E and F, skeletal defects in Sdccag8^ΔC/ΔC mice. Gross appearance of the Sdccag8^ΔC/ΔC face at P0 (E) and digits (F) in Sdccag8^ΔC/+ and Sdccag8^ΔC/ΔC mice at P14. Arrows indicate the cleft palate (E) and polydactyly (F) in Sdccag8^ΔC/ΔC mice. MEF, mouse embryonic fibroblast.