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. 2022 Feb 4;298(3):101686. doi: 10.1016/j.jbc.2022.101686

Figure 5.

Figure 5

Ciliary defects in Sdccag8ΔC/ΔCMEFs.A, immunofluorescent analysis of MEFs from Sdccag8+/+ and Sdccag8ΔC/ΔC mice using anti-Sdccag8 and anti-γ-tubulin antibodies. Nuclei were stained with DAPI. B–D, immunofluorescent analysis of MEFs from Sdccag8+/+ and Sdccag8ΔC/ΔC mice using an anti-acetylated α-tubulin antibody. Nuclei were stained with DAPI (B). The numbers (C) and length (D) of the cilia stained with an antibody against acetylated α-tubulin were measured. Error bars show SD. ∗p < 0.05, ∗∗∗p < 0.001 (unpaired t test), n = 3 mice per genotype. E, qRT-PCR analysis of Gli1 mRNA level in SAG-treated MEFs from Sdccag8+/+ and Sdccag8ΔC/ΔC mice. Shh signal-dependent expression of Gli1 was defective in the Sdccag8ΔC/ΔC MEF. Error bars show SD. ∗p < 0.05, (unpaired t test), n = 3 mice per genotype. MEF, mouse embryonic fibroblast; qRT-PCR, quantitative RT-PCR.