Table E.2. Information extracted from the records retrieved concerning whey proteins to verify the efficacy of HPP on raw milk or raw colostrum according to the criteria described in Section 2.3.2

Compounds	Effect of HPP	Type of milk/colostrum	Stability after the HPP treatment	Effect compared/validated with target pathogens	Methods available to use	References
Whey proteins
β‐lactoglobulin (β‐Lg)	β‐Lg Is the most sensitive whey protein to HPP. Denaturation increased with pressure and longer holding times. Some authors reported denaturation levels above 90% at 600 MPa (Table 6)	Most surveys done with bovine milk, two with goats’ milk (2, 3), one with ovine milk (15) and one with buffalo milk (12). No great differences were reported, although buffalo milk β‐Lg (12) seems more sensitive to HPP than others	No data available	600 MPa for 15 min treatment denatures about 80% of the β‐Lg and reduces at least 7 log10 cycles of L. monocytogenes, S. aureus and Pseudomonas aeruginosa	FPLC (2, 3, 10) RP‐HPLC (1, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15,16, 21) SDS‐PAGE (2, 10, 15, 22), SEC (16) CE (17, 19, 23) RID (18, 20) LC‐MS Q‐ToF (24)	(1) López‐Fandiño et al. (1996); (2) Felipe et al. (1997); (3) Law et al. (1998); (4) Lopez‐Fandiño and Olano (1998); (5) Garcia‐Risco et al. (2000); (6) Scollard et al. (2000a); (7) Huppertz et al. (2004a); (8) Huppertz et al. (2004b); (9) Kiełczewska et al. (2004); (10) Nabhan et al. (2004); (11) Ye et al. (2004); (12) Huppertz et al. (2005); (13) Zobrist et al. (2005); (14) Kleber et al. (2007); (15) Moatsou et al. (2008a); (16) Moatsou et al. (2008b); (17) Bravo et al. (2012); (18) Mazri et al. (2012a); (19) Bravo et al. (2013); (20) Ramos et al. (2015); (21) Leu et al. (2017); (22) Bogahawaththa et al. (2018); (23) Omar et al. (2018); (24) Liu et al. (2020)
Α‐lactalbumin (α‐La)	α‐La is resistant to up to 400 MPa at room temperature. Maximum denaturation level described were 42% (5) in bovine milk and 52.6% in buffalo milk (11) after 30 min treatments at 600 MPa at 20°C (Table 7)	Most surveys done with bovine milk, two with goats’ milk (1,2), one with ovine milk (10), one with buffalo milk (9) and one with camel milk (18). Little interspecies differences were reported.	No data available	No data available	SDS‐PAGE (1, 7, 17) SEC (17) RP‐HPLC (3, 4, 5, 6, 9, 10, 11) CE (12, 14, 18) FPLC (1, 2, 7) RID (13, 15) LC‐MS Q‐ToF (19)	(1) Felipe et al. (1997); (2) Law et al. (1998); (3) Lopez‐Fandiño and Olano (1998); (4) Garcia‐Risco et al. (2000); (5) Huppertz et al. (2004b); (6) Kiełczewska et al. (2004); (7) Nabhan et al. (2004); (8) Ye et al. (2004); (9) Huppertz et al. (2005); (10) Moatsou et al. (2008a); (11) Moatsou et al. (2008b); (12) Bravo et al. (2012); (13) Mazri et al. (2012a); (14) Bravo et al. (2013); (15) Ramos et al. (2015); (16) Leu et al. (2017); (17) Bogahawaththa et al. (2018); (18) Omar et al. (2018); (19) Liu et al. (2020)
Immunoglobulins (Ig)	IgA seems more resistant to denaturation than IgG and IgM Maximum denaturation described after 30 min HPP at 600 MPa and 20°C was 60% for IgA in human colostrum (Table 8)	Two surveys included data from goats’ milk (1,2) and four of human’s milk (5, 6, 7, 11). No differences were described. One survey included data from bovine colostrum (10), one from caprine colostrum (4) and one from human colostrum (8)	No data available	No data available	ELISA (4,6,8, 11,12) FPLC (1, 2) SDS‐PAGE (1) RP‐HPLC (3) RID (4, 9, 10)	(1) Felipe et al. (1997); (2) Law et al. (1998); (3) Kiełczewska et al. (2004); (4) Trujillo et al. (2007); (5) Viazis et al. (2007); (6) Contador et al. (2013); (7) Delgado et al. (2013); (8) Sousa et al. (2014); (9) Ramos et al. (2015); (10) Foster et al. (2016); (11) Mayayo et al. (2016); (12) Bogahawaththa et al. (2018)
Lactoferrin	Dp‐values described in literature: 201 min at 450 MPa; 34.47 min at 600 MPa and 11.37 min at 700 MPa Maximum reported denaturation of 70% after 600 MPa for 15 min (3) (Table 9)	Most surveys done with bovine milk, one survey reported data on human milk (2) and another on camel milk (5).	No data available	No data available	ELISA (1, 2, 3, 4) CE (5)	(1) Mazri et al. (2012b); (2) Mayayo et al. (2014); (3) Ramos et al. (2015); (4) Bogahawaththa et al. (2018); (5) Omar et al. (2018)

CE: capillary electrophoresis; ELISA: enzyme‐linked immunosorbent assay; FPLC: Fast protein liquid chromatography; LC‐MS: liquid chromatography–mass spectrometry; RID: radial immunodiffusion; RP‐HPLC: Reversed‐phase high‐pressure liquid chromatography; SDS‐PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis; SEC: size‐exclusion chromatography.