FIGURE 1.

Sr stimulates the osteogenic ability of hBMSCs and angiogenic ability of HUVECs in vitro. (A) ALP staining of hBMSCs cultured in α-MEM with Sr at 0, 0.2, 1, and 5 mM for 7 days. (B,C) Tube formation assay of HUVECs cultured in ECM with SrCl₂ at 0, 0.2, 1, and 5 mM for 2 h (B) and 4 h (C) with quantitative analysis by ImageJ of (D) mean size of meshes and (E) total mesh area at time point “2 h”. (F) Relative expression of VEGFA gene in HUVECs cultured for 3 days and (G) Ang-1 gene for 7 days in ECM with Sr at 0, 0.2, 1, and 5 mM (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared with the 0 mM group).