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. 2022 Feb 17;10:839062. doi: 10.3389/fchem.2022.839062

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Copyright © 2022 Yan, Li, Wu, Zhang, Hu, Deng, Jiang, Wen and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vivo osseointegration. (A) Bone formation and deposition around the implant samples by polychrome sequential fluorescent labeling method; listed from left to right: alizarin red labeling (red) at 2 weeks and calcein (green) at 6 weeks after surgery of bone tissue and the merged image. (B) Van Gieson’s staining of the sections from rats at 8 weeks after implant surgery. (C) The mean distance between the two labeling lines of alizarin red and calcein (red and green) in (A) was calculated by ImageJ compared with the TiO2 group.(D) Quantification of the bone implant contact (BIC) level of the staining in (B) compared with the TiO2 group.