Fig. 3.

Effect of chronic CORT exposure on the circadian rhythm parameters of CRH in chicken hypothalamus and CRH receptors gene in chicken pituitary. (A) CRH gene; (B) CRHR1 gene; (C) CRHR2 gene. The relative mRNA levels of CRH and CRH receptors gene are normalized to PPIA. The data markers in the graphs indicate the CRH and CRH receptors gene mRNA expression levels, and the results are expressed as the mean ± SEM. The curves represent the 24-h period determined by cosinor analysis. n = 6 chickens per time point. Data from CT2 are double-plotted. R² values represent the degree of fitting. *P < 0.05, compared with control