Table 2.
miRNA primer sequences (5'-3') used in quantitative real-time PCR
|
| ||
| Primer name | Primer sequence (5 3) | |
| miR-Let 7a | ||
| STL | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACATCGT | |
| Forward | GGGTAACACTGTCTGGTAACGAT | |
| miR-145 | ||
| STL | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGGAT | |
| Forward | TTGAACCCTCATCCT GTGAGCC | |
| miR- 451 | ||
| STL | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAA-CTCAG | |
| Forward | GGAAGATCTTGACAAGGAGGACAGGAGAG | |
| Universal | ||
| Reverse | GTGCAGGGTCCGAGGT | |
| U6 | ||
| Forward | GCTTCGGCAGCACATATACTAAAAT | |
| Reverse | CGCTTCACGAATTTGCGTGTCAT | |
| STL | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAAATAT | |
| STL: Stem-loop, Let 7a: Lethal-7, miR: MicroRNA, U6: RNU6-1 | ||