Figure 2.

BGB-A1217 potentiates human T cell response to produce IFN-γ and exhibits combination effects with PD-1 antibody. (A) schematic diagram of CMV assay. Pre-stimulated PBMCs from healthy donors were co-incubated with peptide pulsed HCT116 cells overnight. IFN-γ release was used as readout. (B) secreted IFN-γ in the conditioned media from CMV assay measured by ELISA. Data from two donors (D94 and D122) are shown as mean ± SD. N = 3. (C) schematic diagram of BGB-A1217 and anti-PD-1 combination assay. PBMCs from healthy donors were stimulated with OKT3 (40 ng/mL) for 3 days. Pre-activated PBMCs were co-cultured with a mixture of A549/OS8-PD-L1 and A549/PD-L1 for 18 hours. Cells were incubated with indicated concentrations of BGB-A1217 together with or without 100 ng/mL BGB-A317. (D) Secreted IFN-γ in the conditioned media from combination assay measured by ELISA. Data are shown as mean ± SD. N = 3.