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[Preprint]. 2022 Mar 4:2022.03.02.22271785. [Version 1] doi: 10.1101/2022.03.02.22271785

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Figure 1. — Allelic discrimination plots of deletion assays ORF1aΔ3675-3677 and SΔ69-70. Synthetic RNA controls from six SARS-CoV-2 type strains was amplified in triplicate at 4□10⁴ genome copies/assay via TaqPath RT-qPCR along with no template controls. The deletion probe from the ORF1aΔ3675-3677 assay produced low intensity fluorescence. Sequenced positive saliva samples (n=125) were loaded in duplicate to determine the detection range of the assay in saliva. Data were plotted by using the absolute fluorescence of each reporter dye probe.