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[Preprint]. 2022 Mar 4:2022.03.02.22271785. [Version 1] doi: 10.1101/2022.03.02.22271785

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Figure 2. — Allelic discrimination plots of SNP assays for Spike K417T, E484K, E484Q, L452R. Synthetic RNA controls from six SARS-CoV-2 type strains was amplified in triplicate at 4□10⁴ genome copies/assay via TaqPath RT-qPCR along with no template controls. Synthetic RNA strains that failed to amplify on K417T, E484K, E484Q, or L452R assays lacked both alleles. Sequenced positive saliva samples (n=125) were loaded in duplicate to determine the detection range of the assay in saliva. Data were plotted by using the absolute fluorescence of each reporter dye probe.