Skip to main content
. Author manuscript; available in PMC: 2022 Mar 8.
Published in final edited form as: Acta Biomater. 2020 May 16;111:129–140. doi: 10.1016/j.actbio.2020.04.047

Fig. 6.

Fig. 6.

Osteogenic differentiation. a) Intracellular ALP activity in MC3T3 pre-osteoblasts on 3D-printed scaffolds under ES or non-ES conditions for 14 days. b) OCN content from pre-osteoblasts cultured on 3D-printed scaffolds under ES or non-ES condition for 21 days. RT-qPCR analysis of c) OPN and d) RUNX2 gene expression levels before and after electrical stimulation on each treatment for 14 days. The values within each test were normalized to the 3D-PPFA non-ES group (set as 1). *: p < 0.05 compared to 3D-PPFA group with same non-ES or ES treatment; #: p < 0.05 compared to 3D-PPFA-ssDNA group with same non-ES or ES treatment; $: p < 0.05 compared to 3D-PPFA-CNT group with same non-ES or ES treatment. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)