Figure 4.

DHX16 interacts with RIG-I to enhance IFN-I production

(A) DHX16 interacts with RIG-I following co-expression in HEK293Ts (coIP).

(B) Endogenous DHX16 interacts with ectopic RIG-I in HEK293Ts (coIP).

(C) Diagram of RIG-I truncation mutants.

(D) Interaction between DHX16 and RIG-I domain truncations following co-expression in HEK293Ts (coIP).

(E) Endogenous DHX16 and RIG-I interact in A549s infected with IAV for 24 h (PR8 MOI = 1) (coIP).

(F) IFN-β luciferase reporter activity in HEK293Ts following DHX16 and RIG-I co-expression.

(G) DHX16 and mIFN-β expression from WT or RIG-I KO MEFs following transfection of DHX16 (qRT-PCR and immunoblot).

Data are expressed as means (n = 3) ± SD. ^∗∗p < 0.01; ^∗∗∗∗p < 0.0001 (one-way ANOVA with Tukey's multiple comparisons). Data are representative of 2–3 independent experiments.

See also Figure S3.