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. 2022 Mar 8;38(10):110434. doi: 10.1016/j.celrep.2022.110434

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© 2022 The Authors

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DHX16 interacts with the E3-Ub ligase TRIM6

(A) Interaction between DHX16 and TRIM6 following co-expression in HEK293Ts (coIP).

(B) Diagram of TRIM6 deletion mutants.

(C) Interaction between DHX16 and the SPRY domain of TRIM6 following co-expression in HEK293Ts (coIP).

(D) Co-localization between DHX16 and TRIM6 following co-expression in A549s. Scale bar, 20 μm (confocal microscopy).

(E) Nuclear/cytoplasmic distribution of DHX16 following TRIM6 co-expression in A549s (n = 17).

(F) Interaction between DHX16 and K48-poly-Ub following co-expression with either WT or C15A TRIM6 in HEK293Ts (coIP).

(G) Interaction between endogenous DHX16 and K48-poly-Ub in WT or TRIM6 KO A549s (coIP).

(H) IFN-β, IFITM1, and IFI27 expression from WT or TRIM6 KO A549s transfected with DHX16 for 24 h (qRT-PCR).

Data are expressed as means (n = 3) ± SD. ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001 (one-way or two-way ANOVA with Tukey's multiple comparisons). Data are representative of 2–3 independent experiments.

See also Figures S4 and S5.