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. 2021 Dec 4;4(1):vdab180. doi: 10.1093/noajnl/vdab180

Figure 3.

Figure 3.

EGFRvIII expression is associated with replication stress in human GBM cell samples. Immunohistochemical detection of EGFRvIII, RPA, and γH2AX in human GBM samples with heterogeneous EGFRvIII expression. (A) Representative sample (GBM1). Scale bars: Overview, 10 mm; zoom, 50 µm. Detection of (B) RPA and (C) γH2AX in 5 GBM patient samples. EGFRvIII− areas are depicted on the left, EGFRvIII+ on the right (scale bars: 50 µm). Quantification of percentage of (D) RPA-positive cells and (E) γH2AX-positive cells in the EGFRvIII− and EGFRvIII+ areas (n = 5; mean with SEM; P values are obtained by one-tailed Student’s t-test, *P < .01, ***P < .001). For quantification, 3 fields of view from the EGFRvIII− area and EGFRvIII+ area, respectively, per specimen have been evaluated.