Figure 4.

EGFRvIII expression is associated with DNA double-strand breaks and genomic instability. (A and B) Detection of 53BP1-foci in G1-, S- and G2-phase cells by immunofluorescence. (A) Representative image (pink: 53BP1 foci). For discrimination of different cell cycle phases cells were labeld with EdU (green) before fixation and co-stained with anti-geminin antibodies (red). Cell nuclei were counterstained with DAPI (blue). (B) Quantification of 53BP-foci in G1-, S- and G2-phase cells 72 h after seeding (n = 3). (C) Detection of micronuclei by DAPI. Left: representative image; right: quantification (n = 3). (D and E) Array-based CGH-analysis with (D) genomic instability score (E) CGH-profile.