Fig 6. pNK and YT cells both express GrzmB and H.
(A) Human pNK or YT cells were fixed with 2% PFA, permeabilized, then stained with mouse antibody (1:200) specific for Grzm A followed by a rabbit anti-mouse antibody conjugated to FITC (green histogram). Unstained YT and pNK cells (blue histogram) labelled with isotope IgG1 primary antibody and rabbit anti-mouse antibody conjugated to FITC (red histogram) were used as negative controls. pNK (1x106 cells) or wild type YT cells (5x105 cells) blotted using (1:1000) mouse antibodies specific for human Grzm B (B) or H (C). Rabbit antibody specific for human beta actin antibody was used as a loading control.