Table 2. CRISPR sgRNA sequences.
| CRISPR KO | Target Sequence | Clone | Parent Clone |
|---|---|---|---|
| Grzm B | CCUUCAGGGGAGAUCAUCGG | C8 | WT YT |
| Grzm B | CCUUCAGGGGAGAUCAUCGG | D8 | WT YT |
| Grzm B | CCUUCAGGGGAGAUCAUCGG | E9 | WT YT |
| Grzm H | AACAAAGGCCAUGUAGGGGC | F6 | WT YT |
| Grzm H | AACAAAGGCCAUGUAGGGGC | B9 | WT YT |
| Grzm H | AACAAAGGCCAUGUAGGGGC | F10 | WT YT |
| Granulysin | ACCUCCCCGUCCUACACACC | A9 | WT YT |
| Granulysin | ACCUCCCCGUCCUACACACC | D2 | WT YT |
| Granulysin | ACCUCCCCGUCCUACACACC | E6 | WT YT |
| Perforin | ACAGGGGGACUUGGGCUCU | B3 | WT YT |
| Perforin | ACAGGGGGACUUGGGCUCU | D4 | WT YT |
| Perforin | ACAGGGGGACUUGGGCUCU | F8 | WT YT |
| Grzm B&H | UCCUUCAGGGGAGAUCAUCG | G6 | GrzmH KO F6 YT |
| Grzm B&H | UCCUUCAGGGGAGAUCAUCG | G8 | GrzmH KO F6 YT |
| Grzm B&H | UCCUUCAGGGGAGAUCAUCG | G11 | GrzmH KO F6 YT |
| Grzm B&H | UCCUUCAGGGGAGAUCAUCG | F12 | GrzmH KO F6 YT |